Selection of fish trait modalities in the Minho Estuary (Portugal) that had strong temporal changes (selected by the highest % of variation). Blue lines refer to a simple moving regression (loess) and are only indicative of the temporal changes. Statistical tests can be found in Table 2. fe1 = number of oocites less or equal to 55000, fe2 = number of oocites between 55000 and 60000, inv = invertivorous (feed on invertebrates), ip1 = incubation period is less or equal to seven days, ld2 = larval stage duration between 12 and 25 days, ll1 = larval length is smaller or equal to 4.2 cm, ls1 = life span is less than eight years, ls2 = life span is between eight and fifteen years, ma1 = females are mature before two years, ma4 = females are mature between four and five years, omn = omnivorous (feed on animals and plants), pli = reproduction habitat is phyto-litophilic (associated with plants and rocks), pnh = parental care by protection with nesting or egg hiding, pot = potamodromous migration (between different freshwater bodies), sh1 = shape factor ratio is smaller or equal to 4.35 (compact, rounded body shape), st1 = spawn time is during winter, st2 = spawn time is during summer, sw3 = slow swimmer, wat = feeding habitat is in the water column. The description of all modalities and their units can be found in Appendix 1.