Multiplex PCR assay challenged with DNA from Ganaspis cf. brasiliensis G1 (Gb-1: A01–A05) and G3 (Gb-3: A06–A10), Asobara cf. rufescens (Ar: A11, A12, B11), Leptopilina japonica (China: B01–B05; South Korea: B06–B10), Leptopilina heterotoma (Lh: C01–C05), Pachycrepoideus vindemiae (Pv: C06, C07, D11, D12), Asobara japonica (Aj: C08–C12), Leptopilina maia (Lm: D01–D05), Leptopilina leipsi (Ll: D06–D10) and a negative control (NEG) with no DNA. Alignment markers (in green) are shown at 15 bp and 3000 bp for all samples and positive PCR results are indicated by fragment sizes of 332 bp, 639 bp and 473 bp for Gb-1, Lj and Lh (respectively). Absence of a fragment between the 15 bp and 3000 bp alignment markers indicates a negative PCR result.

 
 
  Part of: Gariepy TD, Abram PK, Adams C, Beal D, Beers E, Beetle J, Biddinger D, Brind'Amour G, Bruin A, Buffington M, Burrack H, Daane KM, Demchak K, Fanning P, Gillett A, Hamby K, Hoelmer K, Hogg B, Isaacs R, Johnson B, Lee JC, Levensen HK, Loeb G, Lovero A, Milnes JM, Park KR, Prade P, Regan K, Renkema JM, Rodriguez-Saona C, Neupane S, Jones C, Sial A, Smythman P, Stout A, Van Timmeren S, Walton VM, Wilson JK, Wang X (2024) Widespread establishment of adventive populations of Leptopilina japonica (Hymenoptera, Figitidae) in North America and development of a multiplex PCR assay to identify key parasitoids of Drosophila suzukii (Diptera, Drosophilidae). NeoBiota 93: 63-90. https://doi.org/10.3897/neobiota.93.121219