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  • ContentsContents
  • Article InfoArticle Info
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  • FigsFigs
  • TabsTabs
  • TaxaTaxa
  • DataData
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Taxa sampling
    • Mock community construction and DNA extraction
      • Test 1: Identifying closely-related species
      • Test 2: Detecting low abundance species
      • Test 3: Mimicking field trap content on species composition
    • PCR amplification
    • Illumina® library preparation
    • MinION library preparation
    • IonTorrent® library preparation
    • False positive detections
    • Reference barcode dataset
    • Illumina® data processing
    • MinION and IonTorrent® data processing
    • Statistical analysis
  • Results
    • Benchmarking of sequencing technologies
    • Mock community analysis
    • Impact of capture and storage conditions on individual detection
  • Discussion
    • Benchmarking of sequencing technologies
    • Impacts of capture and storage conditions on DNA conservation
    • False positives, negatives and unmatched OTUs
    • Biases
  • Conclusion
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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