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  • ContentsContents
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Design of tilapia-specific primers targeting different loci
    • Primer validation (first-stage PCR)
    • Optimization of reaction conditions of the multiplex PCR
    • Primer sensitivity test and comparison with universal fish eDNA primers, tele02
    • Field testing of the optimized protocol
    • Library construction (second-stage PCR), sequencing, and data analysis
  • Results
    • Selection of primer combinations
    • Evaluation of primer specificity, sensitivity, and performance in multiplex PCR amplification
    • Preliminary survey of tilapia invasion in China
  • Discussion
    • Enhanced detection efficiency of tilapia using a multi-locus, taxon-specific multiplex PCR assay
    • Preliminary results on the current status of tilapia invasion in China
  • Conclusion
  • Acknowledgments
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Use of AI
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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