Map of sampling locations for silver carp eDNA within the continent of Africa (A). All sampled sites within southeast Africa (B) are shown as red circles. The two areas sampled are the Olifants system (Olifants and Letaba Rivers, grey inset) and the Komati (Sabie River, red inset). Also shown is the site of introduction and escape of silver carp in South Africa (red star) and the location of the Massingir dam, Mozambique (yellow star). Dotted line delimits the South Africa – Mozambique border.