Map of Czechia with results of the eDNA screening at the sampling locations. Blue lines and areas represent the main water bodies, yellow dots represent each respective sampling point with numbers referring to the sampling sites in Table 2 and Suppl. material 1: Table S1. Pie charts: the red ring around the pie charts indicates unambiguous eDNA detection of A. astaci whereas a white ring represents non-detection. The green colour indicates detection of A. astacus, blue indicates detection of P. leniusculus, yellow represents detection of F. limosus and brown indicates presence of P. virginalis. The neighbouring countries are indicated by their two-letter ISO codes: AT, DE, HU, PL and SK stand for Austria, Germany, Hungary, Poland and Slovakia.