Distribution of populations of invasive crayfish in Czechia screened for Aphanomyces astaci infection between 2017 and 2020. The shape of the symbol distinguishes host species. Populations where the pathogen was detected are marked by symbols with a full red border, those without A. astaci detection by a black dotted border. The fill colour indicates the pathogen genotype group (dark green: group B; yellow: group E). Site no. 25 is the only locality with a known co-occurrence of F. limosus and P. leniusculus, genotype group B was detected in both host species there.