Generalised mixed effect model analysis of the influence of temperature, density and food availability on the amount of detectable eDNA of A. astaci (A) and P. leniusculus (B) in the mesocosm experiment. The amount of detectable eDNA is represented as positive droplets per sample (log scale). A for A. astaci, the eDNA quantity (positive droplets) was significantly higher in tanks with high crayfish density (20 crayfish) at 10 °C, while high temperature (20 °C) had a significant negative effect on the eDNA quantity for all combinations B for P. leniusculus, the eDNA quantity (positive droplets) was significantly higher for the combination “no food” for 20 crayfish at 10 °C, while the combination 20 crayfish provided with food at 20 °C had a significant negative effect on the eDNA quantity.