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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Fungal strains
    • Sampling on elm trees and bark beetle collections
    • DNA extraction from woody samples and insects
    • TaqMan MGB probes and primer design
    • Duplex qPCR assay
    • qPCR specificity and sensitivity assay and standard curve
    • Validation of qPCR assay in plant tissues
    • Linearity and sensitivity of qPCR on DNA from ascospore serial dilution
    • Statistical analysis
  • Results
    • Specificity and sensitivity of qPCR assays
    • Duplex real-time qPCR from plant tissues and bark beetles
    • Linearity and sensitivity of qPCR on DNA from ascospore serial dilution
  • Discussion
  • Acknowledgements
  • References
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