Loop mediated amplification mechanism. Two inner primers consisting of the F3 and forward inner primer (FIP) and two backward primers, the B3 and backward inner primer (BIP) are used to target 6 regions. Additionally, loop primers are often used to accelerate the reaction, denoted here as LF (loop forward) and LB (loop backward) targeting two additional distinct regions. The Bst polymerase displaces each of the DNA strands and initiates synthesis; this leads to the formation of loop structures, which facilitate subsequent rounds of amplification.